Kinesin



3D Structure of Kinesin

The image at the left is a Crystal Structure of the Kif1A Motor Domain Before Mg Release (2zfi)

Kinesin
Kinesins are eukaryotic motor proteins which move along microtubules. Kinesin (KIF) is a dimer consisting of 2 heavy chains and two light chains. The heavy chain contains the globular motor domain (MD), flexible neck linker (FNL) and a long coiled-coil stalk which intertwines to form the dimer. The light chain (KLC) forms the tail region. The KLC contains a cargo binding domain which is called TPR (Tetratricopeptide repeat). The KIFs are named by their gene number. KIF contains a forkhead-associated domain (FHA) which is involved in phosphopeptide recognition. Kinesin protein in yeast is called Kar3. Eg5 or KIF11 is a kinesin (Kinesin-5) which participates in mitosis. KCBP is a Kinesin-like Calmodulin Binding Protein. NOD is a Drosophila chromosome-associated kinesin.

3D Structures of Kinesin
Update June 2011

Kinesin Heavy Chain (KIF)
2wbe – cKIF MD+FNL+Tubulin A+Tubulin B+AMPPNP – cow

1ia0 – KIF1A MD+Tubulin A+Tubulin B+ATP - pig

3gbj – hKIF13B MD+ADP – human

3edl – cKIF MD+ Tubulin A+Tubulin B – EM

2zfi, 2zfj, 2zfk, 2zfl, 2zfm – mKIF1A – mouse

1vfv, 1vfw – mKIF1A MD+Mg-AMPPNP

1vfx - mKIF1A MD+ADP-Mg-AlFx

1vfz - mKIF1A MD+ADP-Mg-VO4

1v8j – mKIF2C MD+Mg-ADP

1i5s - mKIF2C MD (mutant)+Mg-ADP

1i6i - mKIF2C MD (mutant)+Mg-AMPPCP

1v8k - mKIF2C MD+Mg-AMPPNP

2p4n – hKIF+ Tubulin A+TubulinB – EM docking

2vvg – KIF2 MD – Giardia intestinalis

2cjo – hKIF13+inhibitor 30

2nr8 – hKIF9+ADP

2g1l - hKIF1C FHA domain

2owm – NcKIF1C MD – Neurospora crassa

1goj – NcKIF MD

3bfn – hKIF22 MD

3b6u, 3b6v – hKIF3B MD

2rep – hKIFC1 MD

2eh0 - hKIF1B FHA domain – NMR

2edu – hKIF22 C-terminal – NMR

2v14 – hKIF16B

2heh – hKIF2C

2h58 – hKIFC3+ADP

2gry – hKIF2+ADP

2cow – hKIF13B CAP-gly domain – NMR

1mkj – hKIF MD+docked FNL

1bg2 – hKIF MD

3kin, 2kin – KIF MD - rat

2hfx – pKIF1A head+AMPPNP – pig

2hxh - pKIF1A head+ADP

1ry6 – KIF MD – Plasmodium falciparum

2ncd, 1cz7 – DmKIF MD non-claret disjuctional (NCD) dimer – Drosophila melanogaster

1n6m – DmKIF MD+FNL

Kinesin light chain (KLC)
3edt – hKLC2

3ceq, 3nf1 – hKLC2 TPR domain

Kinesin-14
1f9t, 1f9u, 1f9v, 1f9w, 3kar - Kar3 MD (mutant) - yeast

3l1c – DmNCD (mutant)

Eg5
3ken - hKIF11 MD+S-trityl-L-cysteine

2q2y, 2fky, 2q2z, 2g1q, 2fl2, 2fl6, 1yrs - hKIF11+inhibitors

2pg2 - hKIF11+inhibitor 15+ADP

2uyi - hKIF11+inhibitor 33+ADP

2uym - hKIF11+inhibitor 37+ADP

2ieh - hKIF11+inhibitor (R)-mon97

2fme, 3l9h - hKIF11+quinoline inhibitor

1x88, 1q0b - hKIF11 MD+MgADP+monastrol

3k3b - hKIF11 MD+tetrahydro-beta-carboline

3hqd - hKIF11 MD+AMPPNP+Mg

1ii6 - hKIF11 MD+ADP+Mg

3k5e - hKIF11 MD+ADP+enastrol

KCBP
3h4s – KCBP+ADP+Mg – Arabidopsis thaliana

NOD
3dc4 – DmNOD catalytic core domain+ADP

3dcb - DmNOD catalytic core domain+AMPPNP

3dco - DmNOD catalytic core domain+ cTubulin A+cTubulin B